Application

Anti-H2A (H4R3me2s), clone 6G1/1D3, Cat. No. MABE364, is a highly specific mouse monoclonal antibody that targets H2A/H4R3me2s and has been tested in Dot Blot and Western Blotting.

Research CategoryEpigenetics & Nuclear Function

Dot Blot Analysis: 1:500 dilution from a representative lot detected H2A (H4R3me2s) in H2A, H2B, and H4 dot blot.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H2A type 1 (UniProt: P0C0S8; also known as H2A.1, Histone H2A/p) is encoded by the HIST1H2AG (also known as HIST1H2AG, H2AFP, HIST1H2AI, H2AFC) gene (Gene ID: 8329, 8320, 8332, 8336, 8369) in human. Histone H2A is one of four components of the core nucleosomal structure. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Histones undergo a number of post-translational modifications (PTM) in response to various stimuli that serve important functions for regulation of gene expression, DNA repair, and chromatin. Histone H2A, in particular, undergoes acetylation on selected lysines, which may promote unfolding of chromatin and transcription. It can also be phosphorylated on selected serine residues, which mark mitosis and gene repression, or DNA damage. Arginine methylation is a less explored histone post-translational modification. It has been implicated in embryonic development, somatic transcriptional regulation, and cancer development. H2A arginine 3 methylation (R3me1, R3me2s, and R3me2a) is catalyzed by PRMT1 and PRMT5 in vitro. H2A methylation is shown to appear late in oogenesis and is most abundant in the laid egg. Arginine methylations are constrained to pre-mid blastula transition events in the embryo and are considered to be involved in the global transcriptional repression during development. H2A R3me2s is reported to be enriched on nucleosomes containing both active and repressive histone post-translational modifications in human A549 cells and Xenopus embryos. (Ref.: Wilczek, C et al. (2011). J Biol Chem. 286(49):42221-31; Wang, WL et al. (2014). BMC Epigenetics & Chromatin 7:22).

Immunogen

KLH-conjugated linear symmetric di methyl R3 peptide.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell acid extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected H2A (H4R3me2s) in 10 µg of HeLa cell acid extract.

Specificity

Clone 6G1/1D3 specifically detects H2A/H4 arginine 3 symmetric dimethylation (H2A/H4R3me2) in acid extract of HeLa cells.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~15 kDa observed; 14.09 kDa calculated. Uncharacterized bands may be observed in some lysate(s).